产品概述
细胞白介素2（IL-2）是一种主要由有丝分裂中具有抗原活性的T淋巴细胞产生的多效应细胞因子。它在促进抗原特异性T细胞克隆中发挥着重要的作用。另外，已被证明白细胞介素2（IL-2）可以调解各种类型细胞的多种免疫应答。白细胞介素2（IL-2）刺激胸腺细胞增殖，刺激增殖和分化活化的B细胞，促进生长、分化和灭活单核细胞，诱导细胞因子的生产和增长，诱导普通杀伤细胞增加细胞溶解活动，提高淋巴细胞激活的杀伤（LAK细胞）细胞，诱导增殖和分化少突胶质细胞。大鼠白细胞介素2（IL-2）cDNA为含有155个氨基酸残基的前体糖蛋白、包含20个氨基酸残基信号肽，粘合形成成熟蛋白。在氨基酸序列水平，成熟的大鼠白细胞介素2（IL-2）与小鼠白细胞介素2（IL-2）的同源性达80%。
产品原理
本实验采用双抗体夹心ELISA法。抗大鼠IL-2单抗包被于酶标板上，同时加入标本、标准品和生物素化的抗大鼠IL-2抗体，标本、标准品中的IL-2会与加入于孔中的生物素化的抗大鼠IL-2抗体及包被于酶标板上的单抗结合，形成免疫复合物，游离的成分被洗去；加入辣根过氧化物酶标记的亲合素，亲合素与生物素特异性结合，游离的成分被洗去。加入显色底物（显色剂），若反应孔中有IL-2，辣根过氧化物酶会使无色的显色剂变蓝，加终止液变黄。在450nm处测OD值，IL-2浓度与OD450值之间呈正相关，可通过绘制标准曲线求出标本中IL-2浓度。
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